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plasmids containing the yfp (yellow fluorescent protein)gene (pyfp)  (Bio Basic Canada)

 
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    Bio Basic Canada plasmids containing the yfp (yellow fluorescent protein)gene (pyfp)
    Splicing of the small subunit protein S9/uS4 dRPGs is differentially regulated by transcription-associated proteins. ( A ) Pipeline for the identification of paralog-specific repressors <t>of</t> <t>RPS9</t> splicing. The intron of RPS9A was tagged with Mango I aptamer and its associated protein complex was affinity purified on streptavidin beads linked to TO1-Biotin. Sixty-four nonessential nuclear proteins identified by mass spectrometry were deleted in yeast and their effect on splicing examined using <t>YFP</t> splicing reporters containing either the RPS9A or RPS9B introns. ( B ) Enrichment of RPS9A pre-mRNA after pull-down on streptavidin beads relative to input was measured for strain expressing the Mango I-tagged intron (RPS9A-Mango I) and normalized to that measured with the control untagged strain ( RPS9A) for three experiments. ( C ) The relative effect on splicing of the reporters for the introns of RPS9A (iA) or RPS9B (iB) was measured in a strain with a deletion for a known negative regulator of RPS9A splicing ( rps9b Δ) compared to WT in three experiments. ( D ) Deletion of RPS9A interacting protein genes differentially regulates the splicing of RPS9 paralogs. The heatmap indicates the effects on splicing via either the RPS9A or RPS9B intron as indicated; the 21 gene knockouts coding for the RPS9A intron-interacting proteins resulting in a >40% (log 2 0.5) change in the signal of YFP splicing reporters are shown. As indicated by the scale on the right, five gene deletions increased the splicing of the RPS9A reporter by at least 2-fold (log 2 1) and show a difference of 40% (log 2 0.5) or more in splicing of the A reporter over the B reporter, resulting in the selection of these five genes for further analysis. Gene ontology analysis highlighted their common role in transcription regulation, and using a GO term mapper, two more genes were associated with that process and identified with gray dots.
    Plasmids Containing The Yfp (Yellow Fluorescent Protein)gene (Pyfp), supplied by Bio Basic Canada, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/plasmids containing the yfp (yellow fluorescent protein)gene (pyfp)/product/Bio Basic Canada
    Average 90 stars, based on 1 article reviews
    plasmids containing the yfp (yellow fluorescent protein)gene (pyfp) - by Bioz Stars, 2026-06
    90/100 stars

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    1) Product Images from "Transcription factors induce differential splicing of duplicated ribosomal protein genes during meiosis"

    Article Title: Transcription factors induce differential splicing of duplicated ribosomal protein genes during meiosis

    Journal: Nucleic Acids Research

    doi: 10.1093/nar/gkae1321

    Splicing of the small subunit protein S9/uS4 dRPGs is differentially regulated by transcription-associated proteins. ( A ) Pipeline for the identification of paralog-specific repressors of RPS9 splicing. The intron of RPS9A was tagged with Mango I aptamer and its associated protein complex was affinity purified on streptavidin beads linked to TO1-Biotin. Sixty-four nonessential nuclear proteins identified by mass spectrometry were deleted in yeast and their effect on splicing examined using YFP splicing reporters containing either the RPS9A or RPS9B introns. ( B ) Enrichment of RPS9A pre-mRNA after pull-down on streptavidin beads relative to input was measured for strain expressing the Mango I-tagged intron (RPS9A-Mango I) and normalized to that measured with the control untagged strain ( RPS9A) for three experiments. ( C ) The relative effect on splicing of the reporters for the introns of RPS9A (iA) or RPS9B (iB) was measured in a strain with a deletion for a known negative regulator of RPS9A splicing ( rps9b Δ) compared to WT in three experiments. ( D ) Deletion of RPS9A interacting protein genes differentially regulates the splicing of RPS9 paralogs. The heatmap indicates the effects on splicing via either the RPS9A or RPS9B intron as indicated; the 21 gene knockouts coding for the RPS9A intron-interacting proteins resulting in a >40% (log 2 0.5) change in the signal of YFP splicing reporters are shown. As indicated by the scale on the right, five gene deletions increased the splicing of the RPS9A reporter by at least 2-fold (log 2 1) and show a difference of 40% (log 2 0.5) or more in splicing of the A reporter over the B reporter, resulting in the selection of these five genes for further analysis. Gene ontology analysis highlighted their common role in transcription regulation, and using a GO term mapper, two more genes were associated with that process and identified with gray dots.
    Figure Legend Snippet: Splicing of the small subunit protein S9/uS4 dRPGs is differentially regulated by transcription-associated proteins. ( A ) Pipeline for the identification of paralog-specific repressors of RPS9 splicing. The intron of RPS9A was tagged with Mango I aptamer and its associated protein complex was affinity purified on streptavidin beads linked to TO1-Biotin. Sixty-four nonessential nuclear proteins identified by mass spectrometry were deleted in yeast and their effect on splicing examined using YFP splicing reporters containing either the RPS9A or RPS9B introns. ( B ) Enrichment of RPS9A pre-mRNA after pull-down on streptavidin beads relative to input was measured for strain expressing the Mango I-tagged intron (RPS9A-Mango I) and normalized to that measured with the control untagged strain ( RPS9A) for three experiments. ( C ) The relative effect on splicing of the reporters for the introns of RPS9A (iA) or RPS9B (iB) was measured in a strain with a deletion for a known negative regulator of RPS9A splicing ( rps9b Δ) compared to WT in three experiments. ( D ) Deletion of RPS9A interacting protein genes differentially regulates the splicing of RPS9 paralogs. The heatmap indicates the effects on splicing via either the RPS9A or RPS9B intron as indicated; the 21 gene knockouts coding for the RPS9A intron-interacting proteins resulting in a >40% (log 2 0.5) change in the signal of YFP splicing reporters are shown. As indicated by the scale on the right, five gene deletions increased the splicing of the RPS9A reporter by at least 2-fold (log 2 1) and show a difference of 40% (log 2 0.5) or more in splicing of the A reporter over the B reporter, resulting in the selection of these five genes for further analysis. Gene ontology analysis highlighted their common role in transcription regulation, and using a GO term mapper, two more genes were associated with that process and identified with gray dots.

    Techniques Used: Affinity Purification, Mass Spectrometry, Expressing, Control, Selection



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    plasmids containing the yfp (yellow fluorescent protein)gene (pyfp)  (Bio Basic Canada)
    90
    Bio Basic Canada plasmids containing the yfp (yellow fluorescent protein)gene (pyfp)
    Splicing of the small subunit protein S9/uS4 dRPGs is differentially regulated by transcription-associated proteins. ( A ) Pipeline for the identification of paralog-specific repressors <t>of</t> <t>RPS9</t> splicing. The intron of RPS9A was tagged with Mango I aptamer and its associated protein complex was affinity purified on streptavidin beads linked to TO1-Biotin. Sixty-four nonessential nuclear proteins identified by mass spectrometry were deleted in yeast and their effect on splicing examined using <t>YFP</t> splicing reporters containing either the RPS9A or RPS9B introns. ( B ) Enrichment of RPS9A pre-mRNA after pull-down on streptavidin beads relative to input was measured for strain expressing the Mango I-tagged intron (RPS9A-Mango I) and normalized to that measured with the control untagged strain ( RPS9A) for three experiments. ( C ) The relative effect on splicing of the reporters for the introns of RPS9A (iA) or RPS9B (iB) was measured in a strain with a deletion for a known negative regulator of RPS9A splicing ( rps9b Δ) compared to WT in three experiments. ( D ) Deletion of RPS9A interacting protein genes differentially regulates the splicing of RPS9 paralogs. The heatmap indicates the effects on splicing via either the RPS9A or RPS9B intron as indicated; the 21 gene knockouts coding for the RPS9A intron-interacting proteins resulting in a >40% (log 2 0.5) change in the signal of YFP splicing reporters are shown. As indicated by the scale on the right, five gene deletions increased the splicing of the RPS9A reporter by at least 2-fold (log 2 1) and show a difference of 40% (log 2 0.5) or more in splicing of the A reporter over the B reporter, resulting in the selection of these five genes for further analysis. Gene ontology analysis highlighted their common role in transcription regulation, and using a GO term mapper, two more genes were associated with that process and identified with gray dots.
    Plasmids Containing The Yfp (Yellow Fluorescent Protein)gene (Pyfp), supplied by Bio Basic Canada, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/plasmids containing the yfp (yellow fluorescent protein)gene (pyfp)/product/Bio Basic Canada
    Average 90 stars, based on 1 article reviews
    plasmids containing the yfp (yellow fluorescent protein)gene (pyfp) - by Bioz Stars, 2026-06
    90/100 stars
    		  Buy from Supplier
    plasmids containing the yfp (yellow fluorescent protein) gene (pyfp)  (Bio Basic Canada)
    90
    Bio Basic Canada plasmids containing the yfp (yellow fluorescent protein) gene (pyfp)
    Splicing of the small subunit protein S9/uS4 dRPGs is differentially regulated by transcription-associated proteins. ( A ) Pipeline for the identification of paralog-specific repressors <t>of</t> <t>RPS9</t> splicing. The intron of RPS9A was tagged with Mango I aptamer and its associated protein complex was affinity purified on streptavidin beads linked to TO1-Biotin. Sixty-four nonessential nuclear proteins identified by mass spectrometry were deleted in yeast and their effect on splicing examined using <t>YFP</t> splicing reporters containing either the RPS9A or RPS9B introns. ( B ) Enrichment of RPS9A pre-mRNA after pull-down on streptavidin beads relative to input was measured for strain expressing the Mango I-tagged intron (RPS9A-Mango I) and normalized to that measured with the control untagged strain ( RPS9A) for three experiments. ( C ) The relative effect on splicing of the reporters for the introns of RPS9A (iA) or RPS9B (iB) was measured in a strain with a deletion for a known negative regulator of RPS9A splicing ( rps9b Δ) compared to WT in three experiments. ( D ) Deletion of RPS9A interacting protein genes differentially regulates the splicing of RPS9 paralogs. The heatmap indicates the effects on splicing via either the RPS9A or RPS9B intron as indicated; the 21 gene knockouts coding for the RPS9A intron-interacting proteins resulting in a >40% (log 2 0.5) change in the signal of YFP splicing reporters are shown. As indicated by the scale on the right, five gene deletions increased the splicing of the RPS9A reporter by at least 2-fold (log 2 1) and show a difference of 40% (log 2 0.5) or more in splicing of the A reporter over the B reporter, resulting in the selection of these five genes for further analysis. Gene ontology analysis highlighted their common role in transcription regulation, and using a GO term mapper, two more genes were associated with that process and identified with gray dots.
    Plasmids Containing The Yfp (Yellow Fluorescent Protein) Gene (Pyfp), supplied by Bio Basic Canada, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/plasmids containing the yfp (yellow fluorescent protein) gene (pyfp)/product/Bio Basic Canada
    Average 90 stars, based on 1 article reviews
    plasmids containing the yfp (yellow fluorescent protein) gene (pyfp) - by Bioz Stars, 2026-06
    90/100 stars
    		  Buy from Supplier

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    Splicing of the small subunit protein S9/uS4 dRPGs is differentially regulated by transcription-associated proteins. ( A ) Pipeline for the identification of paralog-specific repressors of RPS9 splicing. The intron of RPS9A was tagged with Mango I aptamer and its associated protein complex was affinity purified on streptavidin beads linked to TO1-Biotin. Sixty-four nonessential nuclear proteins identified by mass spectrometry were deleted in yeast and their effect on splicing examined using YFP splicing reporters containing either the RPS9A or RPS9B introns. ( B ) Enrichment of RPS9A pre-mRNA after pull-down on streptavidin beads relative to input was measured for strain expressing the Mango I-tagged intron (RPS9A-Mango I) and normalized to that measured with the control untagged strain ( RPS9A) for three experiments. ( C ) The relative effect on splicing of the reporters for the introns of RPS9A (iA) or RPS9B (iB) was measured in a strain with a deletion for a known negative regulator of RPS9A splicing ( rps9b Δ) compared to WT in three experiments. ( D ) Deletion of RPS9A interacting protein genes differentially regulates the splicing of RPS9 paralogs. The heatmap indicates the effects on splicing via either the RPS9A or RPS9B intron as indicated; the 21 gene knockouts coding for the RPS9A intron-interacting proteins resulting in a >40% (log 2 0.5) change in the signal of YFP splicing reporters are shown. As indicated by the scale on the right, five gene deletions increased the splicing of the RPS9A reporter by at least 2-fold (log 2 1) and show a difference of 40% (log 2 0.5) or more in splicing of the A reporter over the B reporter, resulting in the selection of these five genes for further analysis. Gene ontology analysis highlighted their common role in transcription regulation, and using a GO term mapper, two more genes were associated with that process and identified with gray dots.

    Journal: Nucleic Acids Research

    Article Title: Transcription factors induce differential splicing of duplicated ribosomal protein genes during meiosis

    doi: 10.1093/nar/gkae1321

    Figure Lengend Snippet: Splicing of the small subunit protein S9/uS4 dRPGs is differentially regulated by transcription-associated proteins. ( A ) Pipeline for the identification of paralog-specific repressors of RPS9 splicing. The intron of RPS9A was tagged with Mango I aptamer and its associated protein complex was affinity purified on streptavidin beads linked to TO1-Biotin. Sixty-four nonessential nuclear proteins identified by mass spectrometry were deleted in yeast and their effect on splicing examined using YFP splicing reporters containing either the RPS9A or RPS9B introns. ( B ) Enrichment of RPS9A pre-mRNA after pull-down on streptavidin beads relative to input was measured for strain expressing the Mango I-tagged intron (RPS9A-Mango I) and normalized to that measured with the control untagged strain ( RPS9A) for three experiments. ( C ) The relative effect on splicing of the reporters for the introns of RPS9A (iA) or RPS9B (iB) was measured in a strain with a deletion for a known negative regulator of RPS9A splicing ( rps9b Δ) compared to WT in three experiments. ( D ) Deletion of RPS9A interacting protein genes differentially regulates the splicing of RPS9 paralogs. The heatmap indicates the effects on splicing via either the RPS9A or RPS9B intron as indicated; the 21 gene knockouts coding for the RPS9A intron-interacting proteins resulting in a >40% (log 2 0.5) change in the signal of YFP splicing reporters are shown. As indicated by the scale on the right, five gene deletions increased the splicing of the RPS9A reporter by at least 2-fold (log 2 1) and show a difference of 40% (log 2 0.5) or more in splicing of the A reporter over the B reporter, resulting in the selection of these five genes for further analysis. Gene ontology analysis highlighted their common role in transcription regulation, and using a GO term mapper, two more genes were associated with that process and identified with gray dots.

    Article Snippet: The RNA Mango I tag of the RPS9 intron and plasmids containing the YFP (yellow fluorescent protein)gene (pYFP) or introns of RPS9A or RPS9B inserted in the YFP gene (pY-iA-FP or pY-iB-FP) were generated by gene synthesis (Bio Basic Canada).

    Techniques: Affinity Purification, Mass Spectrometry, Expressing, Control, Selection